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For research uses, RT‐qPCR is typically used not simply to determine the presence or absence of the RNA but to evaluate its concentration. In such cases, a calibration curve needs to be A part of each and every experiment, consisting of at least six serial dilutions of an in vitro−transcribed RNA normal. The starting off focus and fold dilution
For one-column isolation, the Wizard® SV Genomic DNA Purification Technique provides a fast, very simple strategy for the planning of purified and intact DNA from mouse tails, tissues and cultured cells in as minimal as 20 minutes, dependant upon the quantity of samples processed (around 24 by centrifugation, dependant upon the rotor size, or up t
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